Mouse Cardiotrophin-1 / CT-1 ELISA Kit PicoKine®

Mfr.No.

EK0564

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.006	0.117	0.212	0.381	0.637	1.064	1.476	1.917

Description

Mouse Cardiotrophin-1 / CT-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ctf1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cardiotrophin-1; CT-1; Ctf1; CT1; CT 1

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-A203

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CT-1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	270	1852	6181	243	1959	6792
Standard deviation	17.55	79.63	438.85	16.03	84.23	584.11
CV (%)	6.5%	4.3%	7.1%	6.6%	4.3%	8.6%

Protein Function

Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.

Background

Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	270	247	263	274	263	10.3	3.9%
Sample 2	1852	1876	1650	1677	1763	101.06	5.7%
Sample 3	6181	6219	5425	5308	5783	419.01	7.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	13019
Gene Name	Ctf1
UniProt ID	Q60753
Tissue Specificity	Highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels in testis and brain. No expression in spleen.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Cytokines,Diabetes-associated,Energy Metabolism,Energy Transfer Pathways,Hypertrophy,Immunology,Innate Immunity,Lipid Metabolism,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001289	EK0564	96 wells/kit	$499		Add to Cart

Mouse Cardiotrophin-1 / CT-1 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse Cardiotrophin-1 / CT-1 ELISA Kit PicoKine®

Rate it now..!!