Mouse Cadherin-6/CDH6 ELISA Kit PicoKine®

Mfr.No.

EK2097

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.007	0.082	0.137	0.244	0.521	1.084	1.592	2.055

Description

Mouse Cadherin-6/CDH6 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cdh6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cadherin-6; Kidney cadherin; K-cadherin; Cdh6

Immunogen

Expression system for standard: NS0; Immunogen sequence: S54-A615

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Cadherin-6

Subcellular Localization

Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	216	917	2635	242	1071	3643
Standard deviation	10.37	47.68	142.29	12.34	58.91	229.51
CV (%)	4.8%	5.2%	5.4%	5.1%	5.5%	6.3%

Protein Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Background

Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. It is mapped to 15; 15 A1. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	216	249	242	208	228	17.16	7.5%
Sample 2	917	984	1051	1055	1001	56.47	5.6%
Sample 3	2635	2443	2588	2350	2504	113.63	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12563
Antigen	Cadherin-6/CDH6
Gene Name	Cdh6
UniProt ID	P97326
Tissue Specificity	Expressed in fetal, newborn and 7-day-old testis but not in 21-day-old or adult testis.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001643	EK2097	96 wells/kit	$750		Add to Cart

Mouse Cadherin-6/CDH6 ELISA Kit PicoKine®

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