Mouse CA9/Carbonic anhydrase 9 ELISA Kit PicoKine®

Mfr.No.

EK1587

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.042	0.145	0.214	0.345	0.581	1.14	1.612	2.125

Description

Mouse CA9/Ca Ix ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ca9 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Carbonic anhydrase 9;4.2.1.1;Carbonate dehydratase IX;Carbonic anhydrase IX;CA-IX;CAIX;Membrane antigen MN homolog;Ca9;Car9;

Molecular Weight

47265 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-D390

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CA9

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supernatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	34	186	448	35	180	461
Standard deviation	2.55	12.27	28.67	2.73	13.32	35.49
CV (%)	7.5%	6.6%	6.4%	7.8%	7.4%	7.7%

Protein Function

Reversible hydration of carbon dioxide. Participates in pH regulation (By similarity). .

Background

Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	34	35	40	34	35	2.48	7%
Sample 2	186	181	178	165	177	7.76	4.3%
Sample 3	448	417	503	475	460	31.87	6.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	230099
Antigen	CA9/Carbonic
Gene Name	Ca9
UniProt ID	Q8VHB5
Protein Name	Carbonic anhydrase 9

Applications	ELISA
Research Area	Adaptive Immunity,Immunology,T Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001272	EK1587	96 wells/kit	$499		Add to Cart

Mouse CA9/Carbonic anhydrase 9 ELISA Kit PicoKine®

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