Mouse C1R ELISA Kit PicoKine®

Mfr.No.

EK1955

Sensitivity

<0.1 ng/ml

Detection Range

0.625 ng/ml - 40 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.048	0.091	0.179	0.259	0.455	0.818	1.321	1.996

Description

Mouse C1R ELISA Kit PicoKine® (96 Tests). Quantitate Mouse C1ra in cell culture supeatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement C1r-A subcomponent; Complement component 1 subcomponent r-A; C1ra; C1r; Complement C1r-B subcomponent; Complement component 1 subcomponent r-B; C1rb

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-D705

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse C1R

Subcellular Localization

Extracellular space

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1004	3663	23406	1174	4272	24979
Standard deviation	47.19	197.80	1451.17	79.83	196.51	1448.78
CV (%)	4.7%	5.4%	6.2%	6.8%	4.6%	5.8%

Protein Function

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Background

Complement C1r subcomponent?is a?protein?involved in the?complement system?of the?innate immune system.?In humans, C1r is encoded by the?C1R?gene. It is mapped to 6; 6 F2 and 6 F2; 6. C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1004	1190	1038	1174	1101	81.58	7.4%
Sample 2	3663	4171	3599	3605	3759	238.89	6.3%
Sample 3	23406	24315	20399	23979	23024	1550.42	6.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	50909
Gene Name	C1ra
UniProt ID	Q8CG16; Q8CFG9
Tissue Specificity	Specifically expressed in male reproductive tissues.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001452	EK1955	96 wells/kit	$750		Add to Cart

Mouse C1R ELISA Kit PicoKine®

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