Matrices listed below were spiked with certain level of BK and the recovery rates were calculated by comparing the measured value to the expected amount of BK in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
94
EDTA plasma(n=5)
87-105
98
heparin plasma(n=5)
89-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.