Mouse beta IG-H3/TGFBI ELISA Kit PicoKine®

Mfr.No.

EK1191

Sensitivity

<5 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.004	0.036	0.071	0.147	0.321	0.733	1.373	2.009

Description

Mouse beta IG-H3/TGFBI ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tgfbi in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transforming growth factor-beta-induced protein ig-h3;Beta ig-h3;Tgfbi;

Molecular Weight

74597 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G24-H683

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse beta IG-H3

Subcellular Localization

Secreted . Secreted, extracellular space, extracellular matrix . May be associated both with microfibrils and with the cell surface. .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	301	15302	5482	302	1576	5124
Standard deviation	21.37	95.7	241.2	22.65	100.86	312.56
CV (%)	7.1%	6%	4.4%	7.5%	6.4%	6.1%

Protein Function

Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity). .

Background

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	301	315	329	290	308	14.66	4.7%
Sample 2	1595	1475	1467	1429	1491	62.23	4.1%
Sample 3	5482	6340	6191	6242	6063	340.11	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21810
Antigen	IG-H3/TGFBI
Gene Name	Tgfbi
UniProt ID	P82198
Protein Name	Transforming growth factor-beta-induced protein ig-h3
Tissue Specificity	Expressed in heart, kidney, liver, skeletal muscle, testis, thyroid and uterus (PubMed:8024701). .

Applications	ELISA
Research Area	Cytoskeleton/ECM,Developmental Biology,ECM Proteins,Extracellular Matrix,Growth Factors/Hormones,Neuroscience,Organogenesis,Sensory System,Signal Transduction,Structures,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001012	EK1191	96 wells/kit	$499		Add to Cart

Mouse beta IG-H3/TGFBI ELISA Kit PicoKine®

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