Mouse Beta-defensin 4 - DEFB4 ELISA Kit

Mfr.No.

E1204Mo

Description

This sandwich kit is for the accurate quantitative detection of Beta-defensin 4 (also known as DEFB4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BD-4;Beta-defensin 4;DEFB 4;DEFB4;Defensin, beta 4;mBD-4

Reactivity

Mouse

Detection Range

0.05-10ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

7129

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.021ng/ml

Background

Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to mouse (but not human) CCR6 and induce chemotactic activity of CCR6-expressing cells (PubMed: 20068036).

Gene ID	56519
UniProt ID	P82019
Antigen	DEFB4

Research Area

Others

Protocol

These standard curves of E1204Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.283	2.242	2.225
	2.201
3.2ng/ml	1.198	1.213	1.196
	1.228
1.6ng/ml	0.573	0.675	0.658
	0.776
0.8ng/ml	0.288	0.336	0.319
	0.384
0.4ng/ml	0.142	0.16	0.144
	0.179
0ng/ml	0.017	0.017	0
	0.017

Precision

We measured random samples of E1204Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.7	0.13	2.8
Intra-Assay	2	18	1.9	0.12	6.2
Intra-Assay	3	18	1.7	0.11	6.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109069	E1204Mo	48T	$320		Add to Cart
BHE12109069	E1204Mo	96T	$458		Add to Cart

Mouse Beta-defensin 4 - DEFB4 ELISA Kit

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