Mouse B7-1/CD80 ELISA Kit PicoKine®

Mfr.No.

EK0708

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.001	0.082	0.174	0.333	0.609	0.997	1.508	2.112

Description

Mouse B7-1/CD80 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd80 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

T-lymphocyte activation antigen CD80;Activation B7-1 antigen;B7;CD80;Cd80;B7;

Molecular Weight

34590 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D37-K245

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD80

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	153	733	1704	158	717	1630
Standard deviation	9.94	47.64	132.91	13.27	59.51	133.66
CV (%)	6.5%	6.5%	7.8%	8.4%	8.3%	8.2%

Protein Function

Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Background

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1, D37K245, which is composed of two single chains acids with the dipolymer.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	153	139	133	151	144	8.3	5.7%
Sample 2	733	831	739	836	784	48.82	6.2%
Sample 3	1704	1609	1450	1522	1571	95.09	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12519
Antigen	B7-1/CD80
Gene Name	CD80
UniProt ID	Q00609
Protein Name	T-lymphocyte activation antigen CD80
Tissue Specificity	Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.
Sequence	Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunology,Lymphoid,Myeloid,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000580	EK0708	96 wells/kit	$499		Add to Cart

Mouse B7-1/CD80 ELISA Kit PicoKine®

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