Mouse Autophagy Related Protein 10

Mfr.No.

E2643Mo

Sensitivity

3.69ng/L

Detection Range

6-1900ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

5330424L23Rik;5430428K15Rik;Agp10;Apg;APG10;Apg10l;APG10-like;Apg10p;ATG10;Atg10l;Autophagy-related protein 10;mAPG10;Ubiquitin-like-conjugating enzyme ATG10;

Molecular Weight

24577

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2643Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	560.84	30.02	5.35
Intra-Assay	2	18	108.22	8.85	8.18
Intra-Assay	3	18	159.88	9.94	6.22

Background

E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.

Protocol

These standard curves of E2643Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1000ng/L	2.037	2.212	2.153
	2.387
500ng/L	1.52	1.537	1.478
	1.555
250ng/L	1.194	1.092	1.033
	0.99
125ng/L	0.667	0.647	0.588
	0.627
62.5ng/L	0.439	0.45	0.39
	0.46
0ng/L	0.06	0.059	0
	0.059

Gene ID	66795
UniProt ID	Q8R1P4

Research Area	Cell Biology; Neuroscience; Cancer; Cardiovascular; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115398	E2643Mo	48T	$320		Add to Cart
BHE12115398	E2643Mo	96T	$458		Add to Cart

Mouse Autophagy Related Protein 10 - ATG10 ELISA Kit

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