Mouse Aspartyl Asparaginyl Beta-hydroxylase

Mfr.No.

E1863Mo

Sensitivity

0.56ng/L

Detection Range

1-400ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

2310005F16Rik;3110001L23Rik;AI848629;ASP beta-hydroxylase;Aspartate beta-hydroxylase;Aspartyl/asparaginyl beta-hydroxylase;ASPH;AW261690;AW561901;BA;BAH;C79816;cI-3;cI-37;Junc;Peptide-aspartate beta-dioxygenase

Molecular Weight

83042

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1863Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	73.47	4.73	6.44
Intra-Assay	2	18	178.47	4	2.24
Intra-Assay	3	18	116.74	4.33	3.71

Background

specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.

Protocol

These standard curves of E1863Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	2.124	2.032	1.988
	1.941
120ng/L	0.912	0.97	0.926
	1.028
60ng/L	0.57	0.525	0.481
	0.481
30ng/L	0.308	0.3	0.256
	0.293
15ng/L	0.152	0.146	0.102
	0.14
0ng/L	0.041	0.044	0
	0.047

Gene ID	65973
UniProt ID	Q8BSY0

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109675	E1863Mo	48T	$320		Add to Cart
BHE12109675	E1863Mo	96T	$458		Add to Cart

Mouse Aspartyl Asparaginyl Beta-hydroxylase - ASPH ELISA Kit

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