Matrices listed below were spiked with certain level of AQP-3 and the recovery rates were calculated by comparing the measured value to the expected amount of AQP-3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
95
EDTA plasma(n=5)
85-104
95
heparin plasma(n=5)
94-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AQP-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AQP-3 ELISA Kit allows for the in vitro quantitative determination of AQP-3 concentrations in serum, plasma, tissue homogenates and other biological fluids.