Mouse APOH ELISA Kit PicoKine®

Mfr.No.

EK2027

Sensitivity

<50 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.034	0.098	0.150	0.303	0.486	0.951	1.628	2.089

Description

Mouse APOH ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Apoh in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Beta-2-glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Apolipoprotein H; Apo-H; Beta-2-glycoprotein I; B2GPI; Beta (2)GPI; Apoh; B2gp1

Immunogen

Expression system for standard: NS0; Immunogen sequence: G20-C345

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse APOH

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	376	2458	8756	384	2487	8819
Standard deviation	15.04	140.11	402.78	19.58	171.60	617.33
CV (%)	4.0%	5.7%	4.6%	5.1%	6.9%	7.0%

Protein Function

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Background

Apolipoprotein H (Apo-H), previously known as ?2-glycoprotein I and beta-2 glycoprotein I, is a 38 kDa multifunctional apolipoprotein that in humans is encoded by the APOH gene. This gene is mapped to 11 E1; 11 71.8 Cm. Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	376	379	381	366	375	5.76	1.5%
Sample 2	2458	2675	2677	2428	2559	116.98	4.5%
Sample 3	8756	9905	9479	8379	9129	597.13	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11818
Gene Name	Apoh
UniProt ID	Q01339
Protein Name	Beta-2-glycoprotein 1
Tissue Specificity	Expressed by the liver and secreted in plasma.

Applications	ELISA
Research Area	Cardiovascular,Lipid Metabolism,Lipids/Lipoproteins,Metabolism,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001522	EK2027	96 wells/kit	$499		Add to Cart

Mouse APOH ELISA Kit PicoKine®

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