Matrices listed below were spiked with certain level of Ang1 and the recovery rates were calculated by comparing the measured value to the expected amount of Ang1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
96
EDTA plasma(n=5)
88-103
95
heparin plasma(n=5)
93-101
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ang1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Ang1 ELISA Kit allows for the in vitro quantitative determination of Ang1 concentrations in serum, plasma, tissue homogenates and other biological fluids.