Mouse AFP ELISA Kit PicoKine®

Mfr.No.

EK1661

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.006	0.065	0.134	0.269	0.466	0.924	1.552	2.066

Description

Mouse AFP ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Afp in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Afp

Immunogen

Expression system for standard: NS0; Immunogen sequence: K19-V605

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse AFP

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	787	2378	9095	804	2193	8576
Standard deviation	44.07	149.81	436.56	49.84	144.73	471.68
CV (%)	5.6%	6.3%	4.8%	6.2%	6.6%	5.5%

Protein Function

Binds estrogens, fatty acids and metals.

Background

alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and mateal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low mateal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	787	751	817	732	771	32.75	4.2%
Sample 2	2378	2440	2261	2414	2373	68.44	2.8%
Sample 3	9095	10188	9364	8739	9346	534.02	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11576
Gene Name	Afp
UniProt ID	P02772
Protein Name	Alpha-fetoprotein
Tissue Specificity	Plasma.

Applications	ELISA
Research Area	Cancer,Cardiovascular,Developmental Biology,Organogenesis,Serum Proteins,Tumor Antigens,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001537	EK1661	96 wells/kit	$499		Add to Cart

Mouse AFP ELISA Kit PicoKine®

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