Mouse adiponectin,ADP ELISA Kit

Mouse adiponectin,ADP ELISA Kit

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Mfr.No.

CSB-E07272m

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

adiponectin, C1Q and collagen domain containing, ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin, OTTHUMP00000210047, OTTHUMP00000210048, adipocyte, C1Q and collagen domain containing, adipocyte, C1Q and collagen domain-containing, adipo

Recovery

The recovery of goat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	86
	Range %	82-97
1:4	Average %	93
	Range %	85-102
1:8	Average %	95
	Range %	89-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
600	1.974	1.942	1.958	1.857
300	1.331	1.362	1.347	1.246
150	0.912	0.924	0.918	0.817
75	0.587	0.612	0.600	0.499
37.5	0.401	0.388	0.395	0.294
18.75	0.295	0.309	0.302	0.201
9.37	0.214	0.225	0.220	0.119
0	0.102	0.100	0.101

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q60994

Applications	ELISA
Research Area	ADP

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.