Mouse Adenosine Deaminase

Mfr.No.

E1600Mo

Sensitivity

0.023ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of adenosine deaminase (also known as ADA) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

ADA;Adenosine aminohydrolase;Adenosine deaminase

Molecular Weight

39992

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1600Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.15	0.33	4.04
Intra-Assay	2	18	1.09	0.06	5.36
Intra-Assay	3	18	4.23	0.25	6

Background

Adenosine deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme (EC 3.5.4.4) involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the tuover of nucleic acids in tissues. Its primary function in humans is the development and maintenance of the immune system. However, the full physiological role of ADA is not yet completely understood.

Protocol

These standard curves of E1600Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.244	2.222	2.185
	2.201
10ng/ml	1.687	1.703	1.665
	1.72
5ng/ml	1.041	1.053	1.016
	1.066
2.5ng/ml	0.66	0.672	0.634
	0.683
1.25ng/ml	0.433	0.421	0.383
	0.409
0ng/ml	0.028	0.038	0
	0.048

Gene ID	11486
UniProt ID	P03958

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109442	E1600Mo	48T	$320		Add to Cart
BHE12109442	E1600Mo	96T	$458		Add to Cart

Mouse Adenosine Deaminase - ADA ELISA Kit

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