Mouse Acylamino-acid-releasing Enzyme

Mfr.No.

E1553Mo

Description

This sandwich kit is for the accurate quantitative detection of Acylamino-acid-releasing enzyme (also known as APEH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AARE;Acylamino-acid-releasing enzyme;Acylaminoacyl-peptidase;Acyl-peptide hydrolase;APEH;APH

Reactivity

Mouse

Detection Range

0.1-40ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

81581

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.043ng/ml

Background

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser (By similarity).

Gene ID	235606
UniProt ID	Q8R146
Antigen	APEH

Research Area

Cancer; Cell Biology

Protocol

These standard curves of E1553Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.706	1.83	1.781
	1.953
12ng/ml	0.923	0.974	0.924
	1.025
6ng/ml	0.555	0.536	0.487
	0.517
3ng/ml	0.242	0.196	0.146
	0.149
1.5ng/ml	0.099	0.124	0.074
	0.149
0ng/ml	0.051	0.049	0
	0.048

Precision

We measured random samples of E1553Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.72	0.68	4.61
Intra-Assay	2	18	17.92	0.5	2.82
Intra-Assay	3	18	6.73	0.35	5.22

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109400	E1553Mo	48T	$320		Add to Cart
BHE12109400	E1553Mo	96T	$458		Add to Cart

Mouse Acylamino-acid-releasing Enzyme - APEH ELISA Kit

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