Mouse ACE/Cd143 ELISA Kit PicoKine®

Mfr.No.

EK0558

Sensitivity

<10 pg/ml

Detection Range

93.8 pg/ml - 6,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
O.D.	0.084	0.128	0.178	0.253	0.444	0.658	1.511	2.027

Description

Mouse ACE/Cd143 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ace in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Angiotensin-converting enzyme;ACE;3.2.1.-;3.4.15.1;Dipeptidyl carboxypeptidase I;Kininase II;CD143;Angiotensin-converting enzyme, soluble form;Ace;Dcp1;

Molecular Weight

150918 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L35-Q1264

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse ACE

Subcellular Localization

Angiotensin-converting enzyme, soluble form: Secreted .

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	157	733	3455	155	795	3149
Standard deviation	6.9	40.31	272.94	7.59	46.11	286.55
CV (%)	4.4%	5.5%	7.9%	4.9%	5.8%	9.1%

Protein Function

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm. .

Background

Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	157	139	140	158	148	9.01	6%
Sample 2	733	727	809	711	745	37.81	5%
Sample 3	3455	3416	3214	3153	3309	128.57	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11421
Gene Name	ACE
UniProt ID	P09470
Protein Name	Angiotensin-converting enzyme
Tissue Specificity	Testis-specific isoform is expressed in spermatocytes, adult testis.

Applications	ELISA
Research Area	Cancer,Cancer Metabolism,Cardiovascular,Cell Biology,Cofactors, Vitamins/Minerals,Hematopoietic Progenitors,Metabolism,Metabolism Processes,Metalloprotease,Pathways and Processes,Proteolysis/Ubiquitin,Proteolytic Enzymes,Response To Hypoxia,Serum Proteins,Signal Transduction,Stem Cells,Surface Molecules,Vasculature,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000516	EK0558	96 wells/kit	$499		Add to Cart

Mouse ACE/Cd143 ELISA Kit PicoKine®

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