Mouse A Disintegrin and Metalloproteinase with Thrombospondin Motifs 4

Mfr.No.

E1579Mo

Description

This sandwich kit is for the accurate quantitative detection of Mouse A Disintegrin and Metalloproteinase with Thrombospondin Motifs 4 (also known as ADAMTS4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 4;ADAMTS 4;ADAM-TS 4;ADAMTS4;ADAMTS-4;ADAM-TS4;Aggrecanase-1

Reactivity

Mouse

Detection Range

5-300ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

90070

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.91ng/ml

Background

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Cleaves aggrecan at the '392-Glu-|-Ala-393' site (By similarity).

Gene ID	240913
UniProt ID	Q8BNJ2
Antigen	ADAMTS4

Research Area

Cell Biology

Protocol

These standard curves of E1579Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/ml	1.925	1.871	1.831
	1.817
80ng/ml	1.13	1.137	1.097
	1.144
40ng/ml	0.41	0.485	0.445
	0.561
20ng/ml	0.214	0.236	0.196
	0.258
10ng/ml	0.123	0.102	0.062
	0.081
0ng/ml	0.055	0.04	0
	0.025

Precision

We measured random samples of E1579Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	112.7	4.8	4.3
Intra-Assay	2	18	85.9	4.64	5.4
Intra-Assay	3	18	36.9	1.53	4.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109422	E1579Mo	48T	$320		Add to Cart
BHE12109422	E1579Mo	96T	$458		Add to Cart

Mouse A Disintegrin and Metalloproteinase with Thrombospondin Motifs 4 - ADAMTS4 ELISA Kit

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