Monkey primate TNFSF13/APRIL ELISA Kit PicoKine®

Mfr.No.

EK0921-PR

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Monkey

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.009	0.060	0.107	0.195	0.357	0.681	1.255	2.111

Description

Monkey primate TNFSF13/APRIL ELISA Kit PicoKine® (96 Tests). Quantitate Monkey TNFSF13 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor (Ligand) superfamily member 13 transcript variant delta ;Tumor necrosis factor ligand superfamily member 13 ;TNFSF13 ;

Molecular Weight

24279 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A105-L250

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	653	2945	5633	864	3214	5366
Standard deviation	26.8	156.1	219.7	42.4	202.5	268.3
CV (%)	4.1	5.3	3.9	4.9	6.3	5

Background

Tumor necrosis factor ligand superfamily member 13 (TNFSF13) also known as a proliferation-inducing ligand (APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256 (cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alteatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100551507
Gene Name	TNFSF13
UniProt ID	Q2QBA2
Protein Name	Tumor necrosis factor ligand superfamily member 13

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Cancer,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Non-CD,Signal Transduction,TNF Superfamily

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001249	EK0921-PR	96 wells/kit	$499		Add to Cart

Monkey primate TNFSF13/APRIL ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Monkey primate TNFSF13/APRIL ELISA Kit PicoKine®

Rate it now..!!