Monkey primate GDF5/Bmp 14 ELISA Kit PicoKine®

Mfr.No.

EK1504-PR

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Monkey

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.055	0.106	0.161	0.258	0.448	0.797	1.365	2.007

Description

Monkey primate GDF5/Bmp 14 ELISA Kit PicoKine® (96 Tests). Quantitate Monkey Gdf5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth and differentiation factor-5 ;Gdf5 ;gdf-5 ;

Molecular Weight

7491 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A376-R495

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Secreted .

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	196	753	1345	221	834	1416
Standard deviation	10.97	51.2	99.53	16.57	69.22	121.77
CV (%)	6.8	6.8	7.4	7.5	8.3	8.6

Background

Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	705432
Antigen	GDF5/Bmp
Gene Name	Gdf5
UniProt ID	Q8K4X5
Protein Name	Growth and differentiation factor-5

Applications	ELISA
Research Area	Metabolism,Mitochondrial,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001256	EK1504-PR	96 wells/kit	$750		Add to Cart

Monkey primate GDF5/Bmp 14 ELISA Kit PicoKine®

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