Monkey primate CD56/NCAM-1 ELISA Kit PicoKine®

Mfr.No.

EK0706-PR

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Monkey

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.019	0.152	0.286	0.494	0.865	1.493	2.100	2.411

Description

Monkey primate CD56/NCAM-1 ELISA Kit PicoKine® (96 Tests). Quantitate Monkey NCAM1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56;NCAM1;NCAM;

Molecular Weight

94574 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-G718

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/ml)	1.52	3.48	5.34	2.06	4.16	6.27
Standard deviation	0.082	0.212	0.368	0.140	0.312	0.508
CV (%)	5.4	6.1	6.9	6.8	7.5	8.1

Protein Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellacell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	693789
Antigen	CD56/NCAM-1
Gene Name	NCAM1
UniProt ID	P13591
Protein Name	Neural cell adhesion molecule 1
Sequence	Contains 2 fibronectin type-III domains.

Applications	ELISA
Research Area	Axonal Guidance Proteins,Cell Adhesion Proteins,Cell Type Marker,Developmental Biology,ECM Proteins,Growth and Development,Hematopoietic Progenitors,Membrane Proteins,Nervous System Development,Neural Signal Transduction,Neurology Process,Neuron Marker,Neuroscience,Organogenesis,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001244	EK0706-PR	96 wells/kit	$450		Add to Cart

Monkey primate CD56/NCAM-1 ELISA Kit PicoKine®

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