Monkey luteinizing hormone (LH) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027175MK

Sensitivity

0.5 mIU/mL

Detection Range

1 mIU/mL-50 mIU/mL

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

The recovery of human OLAb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-98
EDTA plasma (n=4)	96	91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human OLAb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	87-98
1:2000	Average %	93
	Range %	91-99
1:4000	Average %	95
	Range %	90-102
1:8000	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Monkey

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	LH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Isobaric tags for relative and absolute quantification-based proteomic analysis of testis biopsies in rhesus monkeys treated with transient scrotal hyperthermia Rao M.et al,Oncotarget,Nov 17

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