Matrices listed below were spiked with certain level of LT-B4 and the recovery rates were calculated by comparing the measured value to the expected amount of LT-B4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-99
96
EDTA plasma(n=5)
81-94
87
heparin plasma(n=5)
81-99
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LT-B4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
LT-B4 ELISA Kit allows for the in vitro quantitative determination of LT-B4 concentrations in serum, plasma, tissue homogenates and other biological fluids.