Human Zinc Finger E-Box-binding Homeobox 2

Mfr.No.

E3533Hu

Description

This sandwich kit is for the accurate quantitative detection of human Zinc Finger E-box-binding Homeobox 2 (also known as ZEB2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Smad-interacting protein 1;SMADIP1;ZEB 2;ZEB2;Zinc finger E-box-binding homeobox 2;Zinc finger homeobox protein 1b

Reactivity

Human

Detection Range

0.05-30ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

136447

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.024ng/ml

Background

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed: 16061479, PubMed: 20516212). Represses transcription of E-cadherin (PubMed: 16061479). Represses expression of MEOX2 (PubMed: 20516212).

Gene ID	9839
UniProt ID	O60315
Antigen	ZEB2

Research Area

Others

Protocol

These standard curves of E3533Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.26	2.288	2.24
	2.316
10ng/ml	1.334	1.358	1.31
	1.381
5ng/ml	0.895	0.839	0.791
	0.782
2.5ng/ml	0.493	0.487	0.439
	0.48
1.25ng/ml	0.272	0.265	0.218
	0.259
0ng/ml	0.04	0.048	0
	0.055

Precision

We measured random samples of E3533Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.9	0.13	7
Intra-Assay	2	18	7.3	0.4	5.5
Intra-Assay	3	18	12.9	0.49	3.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105024	E3533Hu	48T	$320		Add to Cart
BHE12105024	E3533Hu	96T	$458		Add to Cart

Human Zinc Finger E-Box-binding Homeobox 2 - ZEB2 ELISA Kit

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