Human Zinc Finger E-Box-binding Homeobox 1

Mfr.No.

E3075Hu

Alternative Names

Negative regulator of IL2;NIL-2-A zinc finger protein;TCF-8;Transcription factor 8;ZEB 1;ZEB1;Zinc finger E-box-binding homeobox 1

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

124074

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.33ng/L

Background

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.

Gene ID	6935
UniProt ID	P37275
Antigen	ZEB1

Research Area

Others

Protocol

These standard curves of E3075Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.001	2.012	1.966
	2.024
800ng/L	0.919	0.998	0.952
	1.076
400ng/L	0.572	0.578	0.532
	0.583
200ng/L	0.255	0.256	0.21
	0.256
100ng/L	0.138	0.139	0.094
	0.141
0ng/L	0.032	0.046	0
	0.06

Precision

We measured random samples of E3075Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	317	18.54	5.8
Intra-Assay	2	18	906.7	26.76	3
Intra-Assay	3	18	1226.9	33.7	2.7

Storage	2-8°C

Reference	The long non-coding RNA ZFAS1 promotes colorectal cancer progression via miR200b/ZEB1 axis Journal: Pathol Res Pract. 2023 Jul;247:154567.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104609	E3075Hu	48T	$320		Add to Cart
BHE12104609	E3075Hu	96T	$458		Add to Cart

Human Zinc Finger E-Box-binding Homeobox 1 - ZEB1 ELISA Kit

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