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Home / ELISA Kit

Human Zinc finger BED domain-containing protein 3 (ZBED3) ELISA Kit

SKU : BHE13702225

Zbed3 contains a PPPPSPT motif, which is crucial to its binding to Axin. The Ser and Thr residues in the motif appear to be also phosphorylated by glycogen synthase kinase 3beta (GSK3beta) and the CKI family kinases, as GSK3beta and CKIepsilon could enhance the interaction of Zbed3 with Axin. Expressing Zbed3, but not these mutants, led to inhibition of GSK3beta-mediated beta-catenin phosphorylation, cytoplasmic beta-catenin accumulation, and activation of lymphoid enhancer binding factor-1-dependent reporter gene transcription. Furthermore, knockdown of Zbed3 with RNA interference attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and the Wnt target gene expression. Zbed3 is a novel Axin-binding protein that is involved in Wnt/beta-catenin signaling modulation.

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Mfr.No.	KTE60005
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Zinc finger BED domain-containing protein 3 (ZBED3) ELISA Kit employs a two-site sandwich ELISA to quantitate ZBED3.
Alternative Names	ZBED3; MGC15435; 2610005H11Rik; zinc finger; BED domain containing 3
Detection Method	Colorimetric
Kit Components	Human Zinc finger BED domain-containing protein 3 microplate Human Zinc finger BED domain-containing protein 3 standard Human Zinc finger BED domain-containing protein 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Zinc finger BED domain-containing protein 3 (ZBED3) ELISA Kit has high sensitivity and excellent specificity for detection of Human ZBED3. No significant cross-reactivity or interference between Human ZBED3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Zbed3 contains a PPPPSPT motif, which is crucial to its binding to Axin. The Ser and Thr residues in the motif appear to be also phosphorylated by glycogen synthase kinase 3beta (GSK3beta) and the CKI family kinases, as GSK3beta and CKIepsilon could enhance the interaction of Zbed3 with Axin. Expressing Zbed3, but not these mutants, led to inhibition of GSK3beta-mediated beta-catenin phosphorylation, cytoplasmic beta-catenin accumulation, and activation of lymphoid enhancer binding factor-1-dependent reporter gene transcription. Furthermore, knockdown of Zbed3 with RNA interference attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and the Wnt target gene expression. Zbed3 is a novel Axin-binding protein that is involved in Wnt/beta-catenin signaling modulation.
Notes	This Human Zinc finger BED domain-containing protein 3 (ZBED3) ELISA Kit employs a two-site sandwich ELISA to quantitate ZBED3 in samples. An antibody specific for ZBED3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZBED3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZBED3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZBED3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	84327

Applications	ELISA
Applications Notes	This Human Zinc finger BED domain-containing protein 3 (ZBED3) ELISA Kit employs a two-site sandwich ELISA to quantitate ZBED3 in samples. An antibody specific for ZBED3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZBED3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZBED3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZBED3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702225	KTE60005	48 T	$339		Add to Cart
BHE13702225	KTE60005	96 T	$589		Add to Cart
BHE13702225	KTE60005	96 Tx5	$2629		Add to Cart
BHE13702225	KTE60005	96 Tx50	$24569		Add to Cart

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