Matrices listed below were spiked with certain level of WT1 and the recovery rates were calculated by comparing the measured value to the expected amount of WT1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
97
EDTA plasma(n=5)
88-103
96
heparin plasma(n=5)
86-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
WT1 ELISA Kit allows for the in vitro quantitative determination of WT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human WT1 (Wilms tumor protein) ELISA Kit