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Home / ELISA Kit

Human Vitamin A (VA) ELISA Kit

SKU : BHE13702294

Vitamin A is a group of unsaturated nutritional organic compounds that includes retinol, retinal, retinoic acid, and several provitamin A carotenoids (most notably beta-carotene). Vitamin A has multiple functions: it is important for growth and development, for the maintenance of the immune system and good vision. Vitamin A is needed by the retina of the eye in the form of retinal, which combines with protein opsin to form rhodopsin, the light-absorbing molecule(4) necessary for both low-light (scotopic vision) and color vision.(5) Vitamin A also functions in a very different role as retinoic acid (an irreversibly oxidized form of retinol), which is an important hormone-like growth factor for epithelial and other cells.

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Mfr.No.	KTE60075
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Vitamin A (VA) ELISA Kit employs a two-site sandwich ELISA to quantitate VA.
Alternative Names	VA
Detection Method	Colorimetric
Kit Components	Human Vitamin A microplate Human Vitamin A standard Human Vitamin A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Vitamin A (VA) ELISA Kit has high sensitivity and excellent specificity for detection of Human VA. No significant cross-reactivity or interference between Human VA and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Vitamin A is a group of unsaturated nutritional organic compounds that includes retinol, retinal, retinoic acid, and several provitamin A carotenoids (most notably beta-carotene). Vitamin A has multiple functions: it is important for growth and development, for the maintenance of the immune system and good vision. Vitamin A is needed by the retina of the eye in the form of retinal, which combines with protein opsin to form rhodopsin, the light-absorbing molecule(4) necessary for both low-light (scotopic vision) and color vision.(5) Vitamin A also functions in a very different role as retinoic acid (an irreversibly oxidized form of retinol), which is an important hormone-like growth factor for epithelial and other cells.
Notes	This Human Vitamin A (VA) ELISA Kit employs a two-site sandwich ELISA to quantitate VA in samples. An antibody specific for VA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Vitamin A (VA) ELISA Kit employs a two-site sandwich ELISA to quantitate VA in samples. An antibody specific for VA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702294	KTE60075	48 T	$359		Add to Cart
BHE13702294	KTE60075	96 T	$619		Add to Cart
BHE13702294	KTE60075	96 Tx5	$2809		Add to Cart
BHE13702294	KTE60075	96 Tx50	$26209		Add to Cart

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