Human Villin-like Protein - VILL ELISA Kit

Mfr.No.

E6399Hu

Alternative Names

VILL;Villin-like protein

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

95907

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.47ng/L

Background

Villin is an actin binding protein expressed mainly in the brush border of the epithelium in vertebrates but sometimes it is ubiquitously expressed in protists and plants. Villin is found localized in the microvilli of the brush border of the epithelium lining of the gut and renal tubules in vertebrates.

Gene ID	50853
UniProt ID	O15195
Antigen	VILL

Research Area

Others

Protocol

These standard curves of E6399Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.373	2.324	2.278
	2.276
600ng/L	1.384	1.297	1.25
	1.209
300ng/L	0.558	0.628	0.582
	0.699
150ng/L	0.322	0.355	0.308
	0.388
75ng/L	0.198	0.163	0.117
	0.129
0ng/L	0.044	0.047	0
	0.049

Precision

We measured random samples of E6399Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	886.1	29.21	3.3
Intra-Assay	2	18	466.83	18.5	3.96
Intra-Assay	3	18	179.81	7.81	4.34

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107761	E6399Hu	48T	$320		Add to Cart
BHE12107761	E6399Hu	96T	$458		Add to Cart

Human Villin-like Protein - VILL ELISA Kit

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