Human Vesicle-associated Membrane Protein 2

Mfr.No.

E4671Hu

Description

This sandwich kit is for the accurate quantitative detection of human Vesicle-associated Membrane Protein 2 (also known as VAMP2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Synaptobrevin-2;VAMP 2;VAMP2;VAMP-2;Vesicle-associated membrane protein 2

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

12663

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

8.36ng/L

Background

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (By similarity) (PubMed: 30929742). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium Channel KCNB1.

Gene ID	6844
UniProt ID	P63027
Antigen	VAMP2

Research Area

Others

Protocol

These standard curves of E4671Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.359	2.33	2.277
	2.302
800ng/L	1.196	1.214	1.161
	1.231
400ng/L	0.783	0.727	0.674
	0.671
200ng/L	0.485	0.483	0.43
	0.48
100ng/L	0.264	0.252	0.199
	0.24
0ng/L	0.051	0.053	0
	0.055

Precision

We measured random samples of E4671Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	379.1	26.03	6.9
Intra-Assay	2	18	847.7	28.47	3.4
Intra-Assay	3	18	693.8	41.45	6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105966	E4671Hu	48T	$320		Add to Cart
BHE12105966	E4671Hu	96T	$458		Add to Cart

Human Vesicle-associated Membrane Protein 2 - VAMP2 ELISA Kit

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