Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine®

Mfr.No.

EK0536

Sensitivity

<4 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.025	0.085	0.164	0.345	0.602	1.078	1.536	2.011

Description

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine® (96 Tests). Quantitate Human PLAUR in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Urokinase plasminogen activator surface receptor;U-PAR;uPAR;Monocyte activation antigen Mo3;CD87;PLAUR;MO3, UPAR;

Molecular Weight

36978 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L23-R303

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Upar

Subcellular Localization

Cell membrane . Cell projection, invadopodium membrane . Colocalized with FAP (seprase) preferentially at the cell surface of invadopodia membrane in a cytoskeleton-, integrin- and vitronectin-dependent manner. .

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	162	487	1379	146	478	1361
Standard deviation	10.69	37.49	66.19	12.11	37.28	85.74
CV (%)	6.6%	7.7%	4.8%	8.3%	7.8%	6.3%

Protein Function

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

Background

The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes. The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator. UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer. PLAUR is located at chromosome 19q13.1-q13.2. The standard product used in this kit is recombinant human uPAR, consisting of 287 amino acids with the molecular mass of 31KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	162	174	158	168	165	6.06	3.6%
Sample 2	487	514	466	509	494	19.09	3.8%
Sample 3	1379	1232	1375	1306	1323	60.02	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5329
Gene Name	PLAUR
UniProt ID	Q03405
Protein Name	Urokinase plasminogen activator surface receptor
Tissue Specificity	Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
Sequence	Contains 3 UPAR/Ly6 domains.

Applications	ELISA
Research Area	Cancer,Cardiovascular,Cell Type Markers,Extracellular Matrix,Immunology,Invasion/Microenvironment,Receptors,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000506	EK0536	96 wells/kit	$499		Add to Cart

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine®

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