Human ULBP3 ELISA Kit PicoKine®

Mfr.No.

EK1686

Sensitivity

<50 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.148	0.189	0.228	0.319	0.573	0.839	1.384	2.077

Description

Human ULBP3 ELISA Kit PicoKine® (96 Tests). Quantitate Human ULBP3 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

UL16-binding protein 3; ALCAN-gamma; NKG2D ligand 3; N2DL-3; NKG2DL3; Retinoic acid early transcript 1N; ULBP3; N2DL3; RAET1N

Immunogen

Expression system for standard: NS0; Immunogen sequence: D30-G217

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ULBP3

Subcellular Localization

Cell membrane. GPI-anchor.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	98	477	1133	101	482	1425
Standard deviation	3.92	20.51	52.12	5.15	26.99	84.08
CV (%)	4.0%	4.3%	4.6%	5.1%	5.6%	5.9%

Protein Function

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.

Background

UL16 binding protein 3 (ULBP3) is a cell surface glycoprotein encoded by ULBP3 gene located on the chromosome 6. It is mapped to 6q25.1. ULBP3 is related to MHC class I molecules, but its gene maps outside the MHC locus. The domain structure of ULBP3 differs significantly from those of conventional MHC class I molecules. It does not contain the ?3 domain and the transmembrane segment. ULBP3 is thus composed of only the ?1?2 domain which is linked to the cell membrane by the GPI anchor. It functions as a stress-induced ligand for NKG2D receptor. The protein encoded by this gene is one of several related ligands of the KLRK1/NKG2D receptor, which is found in primary NK cells. Binding of these ligands to the receptor activates several signal transduction pathways, including the JAK2, STAT5, and ERK pathways. The encoded protein is expressed solubly and on the surface of many tumor cells, making it potentially an important target for therapeutics.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	98	98	102	85	95	6.41	6.7%
Sample 2	477	459	496	467	474	13.82	2.9%
Sample 3	1133	1050	1116	1070	1092	33.55	3.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	79465
Gene Name	ULBP3
UniProt ID	Q9BZM4

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001553	EK1686	96 wells/kit	$499		Add to Cart

Human ULBP3 ELISA Kit PicoKine®

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