Human Ubiquitin Thioesterase Otub2

Mfr.No.

E6374Hu

Alternative Names

C14orf137;Deubiquitinating enzyme OTUB2;OTB2;OTU domain-containing ubiquitin aldehyde-binding protein 2;OTU2;OTUB2;Otubain-2;Ubiquitin thioesterase OTUB2;Ubiquitin-specific-processing protease OTUB2;

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27213

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.31ng/L

Background

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

Gene ID	78990
UniProt ID	Q96DC9
Antigen	OTUB2

Research Area

Cell Biology

Protocol

These standard curves of E6374Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.852	1.855	1.827
	1.858
800ng/L	0.992	0.965	0.937
	0.938
400ng/L	0.647	0.571	0.543
	0.494
200ng/L	0.147	0.188	0.16
	0.229
100ng/L	0.063	0.059	0.031
	0.055
0ng/L	0.028	0.028	0
	0.028

Precision

We measured random samples of E6374Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	968.43	49.45	5.11
Intra-Assay	2	18	1362.78	42.61	3.13
Intra-Assay	3	18	351.09	17.97	5.12

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107736	E6374Hu	48T	$320		Add to Cart
BHE12107736	E6374Hu	96T	$458		Add to Cart

Human Ubiquitin Thioesterase Otub2 - OTUB2 ELISA Kit

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