Human Ubiquitin Specific Peptidase 8

Mfr.No.

E2338Hu

Alternative Names

DEN1;Deneddylase-1;NEDD8-specific protease 1;NEDP1;Protease, cysteine 2;PRSC2;Sentrin/SUMO-specific protease SENP8;Sentrin-specific protease 8;USP8;

Reactivity

Human

Detection Range

0.05-15ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

24107

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.021ng/ml

Background

Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.

Gene ID	123228
UniProt ID	Q96LD8
Antigen	USP8

Research Area

Cell Biology

Protocol

These standard curves of E2338Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.135	2.212	2.182
	2.289
4ng/ml	1.435	1.391	1.361
	1.347
2ng/ml	0.648	0.652	0.622
	0.657
1ng/ml	0.318	0.381	0.35
	0.443
0.5ng/ml	0.215	0.196	0.166
	0.177
0ng/ml	0.045	0.03	0
	0.015

Precision

We measured random samples of E2338Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.29	0.19	3.62
Intra-Assay	2	18	1.82	0.1	5.61
Intra-Assay	3	18	3.25	0.15	4.62

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103876	E2338Hu	48T	$320		Add to Cart
BHE12103876	E2338Hu	96T	$458		Add to Cart

Human Ubiquitin Specific Peptidase 8 - USP8 ELISA Kit

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