Human Ubiquitin Carboxyl Terminalhydrolase L1

Mfr.No.

E2328Hu

Sensitivity

0.05ng/ml

Detection Range

0.1-38ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Ubiquitin Carboxyl Terminal Hydrolase L1 (also known as UCHL1) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Deubiquitinating enzyme 1;hUBP;Ubiquitin carboxyl-terminal hydrolase 1;Ubiquitin thioesterase 1;Ubiquitin-specific-processing protease 1;USP 1;USP1

Molecular Weight

88207

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2328Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2	0.16	7.8
Intra-Assay	2	18	12	0.53	4.4
Intra-Assay	3	18	3.8	0.28	7.4

Background

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed: 15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed: 16531995). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed: 18082604, PubMed: 26388029).

Protocol

These standard curves of E2328Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.245	2.235	2.205
	2.224
10ng/ml	1.715	1.641	1.611
	1.566
5ng/ml	0.992	1.038	1.008
	1.083
2.5ng/ml	0.684	0.677	0.648
	0.67
1.25ng/ml	0.417	0.448	0.419
	0.479
0ng/ml	0.028	0.029	0
	0.03

Gene ID	7398
UniProt ID	O94782

Research Area	Others

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12103866	E2328Hu	48T	$320		Add to Cart
BHE12103866	E2328Hu	96T	$458		Add to Cart

Human Ubiquitin Carboxyl Terminalhydrolase L1 - UCHL1 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Ubiquitin Carboxyl Terminalhydrolase L1 - UCHL1 ELISA Kit

Rate it now..!!