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Human Ubiquitin Carboxyl-terminalhydrolase Isozyme L1 - UCHL1 ELISA Kit

BHE12115566

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a Peptide bond at the C-terminal glycine of ubiquitin (PubMed: 9774100, PubMed: 8639624, PubMed: 12408865, PubMed: 23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed: 12408865).

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Gene ID 7345
UniProt ID P09936
Antigen UCHL1
Research Area Cell Biology
Protocol These standard curves of E6865Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.639 2.486 2.457
2.333
10ng/ml 1.574 1.567 1.538
1.56
5ng/ml 0.873 0.848 0.819
0.824
2.5ng/ml 0.45 0.472 0.443
0.493
1.25ng/ml 0.228 0.24 0.211
0.252
0ng/ml 0.029 0.029 0
0.029
Precision We measured random samples of E6865Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 10.8 0.43 4
Intra-Assay 2 18 3.6 0.16 4.4
Intra-Assay 3 18 6.5 0.33 5
Storage 2-8°C
SKU Supplier No. Size Your price Quantity
BHE12115566 E6865Hu 48T $320
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BHE12115566 E6865Hu 96T $458
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