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Home / ELISA Kit

Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) ELISA Kit

SKU : BHE13702298

Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains.
During analysis of randomly sampled human coding sequences from the myeloid cell line KG-1, Nomura et al. (1994) identified a novel cDNA, which they referred to as KIAA0055, that was related to the tre2 oncogene. Janssen et al. (1998) found that HUMORF8 encodes a putative deubiquitinating enzyme. Janssen et al. (1998) analyzed DNA from a patient with chronic myeloproliferative disorder. They identified an oncogenic fusion of the 5-prime end of p85-beta with the 3-prime end of HUMORF8.

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Mfr.No.	KTE60079
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) ELISA Kit employs a two-site sandwich ELISA to quantitate USP8.
Alternative Names	USP8; FLJ34456; HumORF8; KIAA0055; MGC129718; UBPY; ubiquitin specific protease 8
Detection Method	Colorimetric
Kit Components	Human Ubiquitin carboxyl-terminal hydrolase 8 microplate Human Ubiquitin carboxyl-terminal hydrolase 8 standard Human Ubiquitin carboxyl-terminal hydrolase 8 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) ELISA Kit has high sensitivity and excellent specificity for detection of Human USP8. No significant cross-reactivity or interference between Human USP8 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. During analysis of randomly sampled human coding sequences from the myeloid cell line KG-1, Nomura et al. (1994) identified a novel cDNA, which they referred to as KIAA0055, that was related to the tre2 oncogene. Janssen et al. (1998) found that HUMORF8 encodes a putative deubiquitinating enzyme. Janssen et al. (1998) analyzed DNA from a patient with chronic myeloproliferative disorder. They identified an oncogenic fusion of the 5-prime end of p85-beta with the 3-prime end of HUMORF8.
Notes	This Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) ELISA Kit employs a two-site sandwich ELISA to quantitate USP8 in samples. An antibody specific for USP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9101

Applications	ELISA
Applications Notes	This Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) ELISA Kit employs a two-site sandwich ELISA to quantitate USP8 in samples. An antibody specific for USP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702298	KTE60079	48 T	$339		Add to Cart
BHE13702298	KTE60079	96 T	$589		Add to Cart
BHE13702298	KTE60079	96 Tx5	$2629		Add to Cart
BHE13702298	KTE60079	96 Tx50	$24569		Add to Cart

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