Matrices listed below were spiked with certain level of U-II and the recovery rates were calculated by comparing the measured value to the expected amount of U-II in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
96
EDTA plasma(n=5)
89-105
98
heparin plasma(n=5)
88-95
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of U-II and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
U-II ELISA Kit allows for the in vitro quantitative determination of U-II concentrations in serum, plasma, tissue homogenates and other biological fluids.