Human Tumor Necrosis Factor betaeta

Mfr.No.

E0081Hu

Alternative Names

LT-alpha;Lymphotoxin-alpha;TNF B;TNF beta;TNFB;TNF-beta;Tumor necrosis factor ligand superfamily member 1

Reactivity

Human

Detection Range

2-600ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

22297

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.05ng/L

Background

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed: 9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

Gene ID	4049
UniProt ID	P01374
Antigen	TNFB

Research Area

Immunology; Signal Transduction; Cancer

Protocol

These standard curves of E0081Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	1.948	1.959	1.927
	1.97
160ng/L	1.497	1.53	1.498
	1.563
80ng/L	0.677	0.683	0.651
	0.689
40ng/L	0.369	0.338	0.306
	0.307
20ng/L	0.176	0.171	0.139
	0.165
0ng/L	0.03	0.032	0
	0.033

Precision

We measured random samples of E0081Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	90.6	3.14	3.5
Intra-Assay	2	18	169.4	7.02	4.1
Intra-Assay	3	18	70	3.11	4.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101816	E0081Hu	96T	$458		Add to Cart
BHE12101816	E0081Hu	48T	$320		Add to Cart

Human Tumor Necrosis Factor betaeta - TNF-B ELISA Kit

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