Human Tubulin Alpha-3E Chain - TUBA3E ELISA Kit

Mfr.No.

E6332Hu

Alternative Names

Alpha-tubulin 3E;Detyrosinated tubulin alpha-3E chain;TUBA 3E;TUBA3E;Tubulin alpha-3E chain

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

49859

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.35ng/L

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

Gene ID	112714
UniProt ID	Q6PEY2
Antigen	TUBA3E

Research Area

Others

Protocol

These standard curves of E6332Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.579	2.586	2.539
	2.593
800ng/L	1.406	1.379	1.332
	1.352
400ng/L	0.809	0.833	0.785
	0.857
200ng/L	0.512	0.458	0.41
	0.404
100ng/L	0.214	0.216	0.168
	0.218
0ng/L	0.043	0.047	0
	0.052

Precision

We measured random samples of E6332Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	919.8	28.87	3.1
Intra-Assay	2	18	694.9	32.76	4.7
Intra-Assay	3	18	333.2	20.7	6.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107694	E6332Hu	48T	$320		Add to Cart
BHE12107694	E6332Hu	96T	$458		Add to Cart

Human Tubulin Alpha-3E Chain - TUBA3E ELISA Kit

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