Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®

Mfr.No.

EK0898

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.068	0.113	0.212	0.301	0.459	0.714	1.236	1.861

Description

Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine® (96 Tests). Quantitate Human Tryptase/TPSAB1,B2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Molecular Weight

30515 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Met1-Pro275

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Tryptase/TPSAB1,B2

Subcellular Localization

Secreted. Released from the secretory granules upon mast cell activation.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	276	1887	5608	283	2054	5485
Standard deviation	18.49	143.41	420.6	23.48	191.02	488.16
CV (%)	6.7%	7.6%	7.5%	8.3%	9.3%	8.9%

Protein Function

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. .

Background

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	276	255	284	276	272	10.75	3.9%
Sample 2	1887	1991	1959	1839	1919	59.59	3.1%
Sample 3	5608	5276	5559	5824	5566	195.24	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7177
Gene Name	Tryptase/TPSAB1,B2
UniProt ID	Q15661
Tissue Specificity	TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin; in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).

Applications	ELISA
Research Area	Cell Biology,Immunology,Innate Immunity,Interspecies Interaction,Microbiology,Proteolysis/Ubiquitin,Proteolytic Enzymes,Secreted Molecules

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001063	EK0898	96 wells/kit	$450		Add to Cart

Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®

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