Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0898

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.068	0.113	0.212	0.301	0.459	0.714	1.236	1.861

Description

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human Tryptase/TPSAB1,B2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA).

Alternative Names

Tryptase beta-2;Tryptase-2;3.4.21.59;Tryptase II;TPSB2;TPS2;

Molecular Weight

30515 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-V275

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Tryptase/TPSAB1,B2

Subcellular Localization

Secreted. Released from the secretory granules upon mast cell activation.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	895	2837	6370	1384	3015	8572
Standard deviation	44.75	175.89	293.02	78.89	211.05	582.9
CV (%)	5%	6.2%	4.6%	5.7%	7%	6.8%

Protein Function

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. .

Background

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	7177
Gene Name	Tryptase/TPSAB1,B2
UniProt ID	Q15661
Tissue Specificity	TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin; in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).

Applications	ELISA
Research Area	Cell Biology,Immunology,Innate Immunity,Interspecies Interaction,Microbiology,Proteolysis/Ubiquitin,Proteolytic Enzymes,Secreted Molecules

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000018	EZ0898	5 plates/kit	$500		Add to Cart

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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