Human Tropomyosin Alpha-4 Chain

Mfr.No.

E6325Hu

Sensitivity

7.54ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

TM30p1;TPM 4;TPM4;Tropomyosin alpha-4 chain;Tropomyosin-4

Molecular Weight

28522

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6325Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	201.4	11.56	5.7
Intra-Assay	2	18	582.8	28.33	4.9
Intra-Assay	3	18	983.2	32.38	3.3

Background

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments (By similarity). Binds calcium (PubMed: 1836432).

Protocol

These standard curves of E6325Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.278	2.321	2.306
	2.363
800ng/L	1.297	1.32	1.306
	1.343
400ng/L	0.788	0.672	0.657
	0.555
200ng/L	0.436	0.396	0.381
	0.356
100ng/L	0.13	0.13	0.116
	0.13
0ng/L	0.015	0.015	0
	0.015

Gene ID	7171
UniProt ID	P67936

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107687	E6325Hu	48T	$320		Add to Cart
BHE12107687	E6325Hu	96T	$458		Add to Cart

Human Tropomyosin Alpha-4 Chain - TPM4 ELISA Kit

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