Human Tropomyosin Alpha-1 Chain

Mfr.No.

E3610Hu

Sensitivity

7.29ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Alpha-tropomyosin;TPM 1;TPM1;Tropomyosin alpha-1 chain;Tropomyosin-1

Molecular Weight

32709

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3610Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	539.1	29.44	5.5
Intra-Assay	2	18	1108.2	34.07	3.1
Intra-Assay	3	18	234.9	14.87	6.3

Background

Binds to actin filaments in muscle and non-muscle cells (PubMed: 23170982). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (PubMed: 23170982). Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Protocol

These standard curves of E3610Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.386	2.349	2.294
	2.313
800ng/L	1.272	1.276	1.221
	1.28
400ng/L	0.695	0.709	0.655
	0.724
200ng/L	0.495	0.455	0.4
	0.414
100ng/L	0.236	0.274	0.219
	0.312
0ng/L	0.043	0.055	0
	0.067

Gene ID	7168
UniProt ID	P09493

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105087	E3610Hu	48T	$320		Add to Cart
BHE12105087	E3610Hu	96T	$458		Add to Cart

Human Tropomyosin Alpha-1 Chain - TPM1 ELISA Kit

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