Human Trophoblast Glycoprotein

Mfr.No.

E6324Hu

Alternative Names

5T4 oncofetal antigen;5T4 oncofetal trophoblast glycoprotein;5T4 oncotrophoblast glycoprotein;M6P1;TPBG;Trophoblast glycoprotein;WAIF1;Wnt-activated inhibitory factor 1

Reactivity

Human

Detection Range

0.05-30ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

46032

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.022ng/mL

Background

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Gene ID	7162
UniProt ID	Q13641
Antigen	TPBG

Research Area

Stem Cells

Protocol

These standard curves of E6324Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/mL	1.901	1.874	1.828
	1.847
8ng/mL	1.049	0.965	0.918
	0.881
4ng/mL	0.613	0.628	0.581
	0.642
2ng/mL	0.254	0.24	0.193
	0.225
1ng/mL	0.092	0.117	0.071
	0.143
0ng/mL	0.054	0.046	0
	0.039

Precision

We measured random samples of E6324Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.2	0.34	2.8
Intra-Assay	2	18	4.4	0.24	5.4
Intra-Assay	3	18	7.6	0.31	4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107686	E6324Hu	48T	$320		Add to Cart
BHE12107686	E6324Hu	96T	$458		Add to Cart

Human Trophoblast Glycoprotein - TPBG ELISA Kit

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