Human TRKC ELISA Kit PicoKine®

Mfr.No.

EK1676

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.036	0.062	0.124	0.197	0.319	0.579	1.049	1.947

Description

Human TRKC ELISA Kit PicoKine® (96 Tests). Quantitate Human NTRK3 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

NT-3 growth factor receptor; GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; NTRK3; TRKC

Immunogen

Expression system for standard: NS0; Immunogen sequence: C32-A428

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TRKC

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	330	1741	4941	335	1753	4967
Standard deviation	18.15	85.3	390.33	22.78	92.9	466.89
CV (%)	5.5%	4.9%	7.9%	6.8%	5.3%	9.4%

Protein Function

Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.

Background

Tropomyosin receptor kinase C? (TrkC),?also known as?NT-3 growth factor receptor,?neurotrophic tyrosine kinase receptor type 3, or?TrkC tyrosine kinase?is a?protein?that in humans is encoded by the?NTRK3?gene.This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene.?

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	330	342	297	342	327	18.41	5.6%
Sample 2	1741	1582	1616	1731	1667	69.63	4.1%
Sample 3	4941	4243	4806	4622	4653	262.39	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4916
Gene Name	NTRK3
UniProt ID	Q16288
Tissue Specificity	Widely expressed but mainly in nervous tissue. Isoform 2 is expressed at higher levels in adult brain than in fetal brain.

Applications	ELISA
Research Area	Cancer,Growth and Development,Metabolism,Metabolism Processes,Neurology Process,Neuroscience,Oncoproteins,Oncoproteins/Suppressors,Pathways and Processes

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001349	EK1676	96 wells/kit	$750		Add to Cart

Human TRKC ELISA Kit PicoKine®

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