Human Tripeptidyl Peptidase 1

Mfr.No.

E2520Hu

Sensitivity

3.19ng/L

Detection Range

7-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Cell growth-inhibiting gene 1 protein;LPIC;Lysosomal pepstatin-insensitive protease;TPP 1;TPP1;TPP-1;TPP-I;Tripeptidyl aminopeptidase;Tripeptidyl-peptidase 1;Tripeptidyl-peptidase I

Molecular Weight

61248

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2520Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	274.7	16.36	6
Intra-Assay	2	18	501.1	18.89	3.8
Intra-Assay	3	18	97.7	5.43	5.6

Background

Lysosomal serine protease with tripeptidyl-peptidase I activity (PubMed: 11054422, PubMed: 19038966, PubMed: 19038967). May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases (PubMed: 11054422, PubMed: 19038966, PubMed: 19038967). Requires substrates with an unsubstituted N-terminus (PubMed: 19038966).

Protocol

These standard curves of E2520Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.655	2.571	2.52
	2.487
400ng/L	1.468	1.483	1.432
	1.498
200ng/L	0.867	0.842	0.791
	0.817
100ng/L	0.39	0.385	0.334
	0.379
50ng/L	0.271	0.239	0.188
	0.207
0ng/L	0.041	0.051	0
	0.061

Gene ID	1200
UniProt ID	O14773

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104058	E2520Hu	48T	$320		Add to Cart
BHE12104058	E2520Hu	96T	$458		Add to Cart

Human Tripeptidyl Peptidase 1 - TPP1 ELISA Kit

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