Human TREML2 ELISA Kit PicoKine®

Mfr.No.

EK1985

Sensitivity

<50 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.018	0.065	0.123	0.230	0.424	0.716	1.195	1.992

Description

Human TREML2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TREML2 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Trem-like transcript 2 protein; TLT-2; Triggering receptor expressed on myeloid cells-like protein 2; TREML2; C6orf76, TLT2; UNQ6268/PRO20473

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G19-S268

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TREmL2

Subcellular Localization

Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	94	472	1928	111	507	1976
Standard deviation	6.30	24.54	109.90	6.99	27.38	81.02
CV (%)	6.7%	5.2%	5.7%	6.3%	5.4%	4.1%

Protein Function

Cell surface receptor that may play a role in the innate and adaptive immune response. Acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation.

Background

TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties. This gene is mapped to 6p21.1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	94	101	88	94	94	4.60	4.8%
Sample 2	472	395	417	458	435	30.90	7.1%
Sample 3	1928	1838	1770	2074	1902	113.77	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	79865
Gene Name	TREML2
UniProt ID	Q5T2D2
Tissue Specificity	Detected in cultured B-cells, T-cell leukemia and monocyte leukemia. Expressed constitutively on CD8 T-cells and induced on CD4 T-cells after activation.

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Immunology,Innate Immunity,Macrophage/Inflammation,Non-CD,T Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001487	EK1985	96 wells/kit	$750		Add to Cart

Human TREML2 ELISA Kit PicoKine®

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