Human Transthyretin/TTR ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1684

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.05	0.083	0.107	0.135	0.232	0.505	1.079	2.263

Description

Human Transthyretin/TTR ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TTR in cell culture supeatants, serum and plasma (heparin, EDTA).

Alternative Names

Transthyretin; ATTR; Prealbumin; TBPA; TTR; PALB

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G21-E147

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Transthyretin/TTR

Subcellular Localization

Secreted. Cytoplasm.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1.94	5.59	11.4	2.08	5.84	12.7
Standard deviation	0.091	0.313	0.73	0.114	0.362	0.914
CV (%)	4.7%	5.6%	6.4%	5.5%	6.2%	7.2%

Protein Function

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

Background

Transthyretin (TTR) is also known as prealbumin. It is mapped to chromosome region 18q11.2-q12.1. This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	7276
Gene Name	TTR
UniProt ID	P02766
Tissue Specificity	Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

Applications	ELISA
Research Area	Alzheimer's Disease,Cardiovascular,Neurodegenerative Disease,Neurology Process,Neuroscience,Serum Proteins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000066	EZ1684	5 plates/kit	$500		Add to Cart

Human Transthyretin/TTR ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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